Systems pharmacology of hepatic metabolism in zebrafish larvae

Programming effects of high-carbohydrate feeding of larvae on adult glucose metabolism in zebrafish, Danio rerio.

The aim of the present study was to determine the potential long-term metabolic effects of early nutritional programming on carbohydrate utilisation in adult zebrafish (Danio rerio). High-carbohydrate diets were fed to fish during four ontogenetic stages: from the first-feeding stage to the end of the yolk-sac larval stage; from the first-feeding stage to 2 d after yolk-sac exhaustion; after yo...

Metabolism Pharmacology

Automatic classification of behavior in zebrafish larvae

Zebrafish larvae navigate the environment by discrete episode of propulsion called bouts. We introduce a novel method for automatically classifying tail bouts. A supervised soft-clustering algorithm to categorize tail bouts into 5 categories of movements: Scoot, Asymmetrical Scoot, Routine Turn, C Bend and Burst. Tail bouts were correctly classified with 82% chance while errors in the classific...

Automated analysis of behavior in zebrafish larvae.

Zebrafish larvae have become a popular model system to examine genetic and environmental factors that affect behavior. However, studying complex behavior in large numbers of fish larvae can be challenging. The present study describes a novel high-resolution imaging system that is unique in its ability to automatically analyze the location and orientation of zebrafish larvae in multiwell plates....

Velocity storage mechanism in zebrafish larvae.

The optokinetic reflex (OKR) and the angular vestibulo-ocular reflex (aVOR) complement each other to stabilize images on the retina despite self- or world motion, a joint mechanism that is critical for effective vision. It is currently hypothesized that signals from both systems integrate, in a mathematical sense, in a network of neurons operating as a velocity storage mechanism (VSM). When exp...